Type I Collagen Matrix and .BETA.-glycerophosphate Facilitate Mineralized Tissue Formation by Rat Dental Pulp Cells.
نویسندگان
چکیده
منابع مشابه
Tissue engineering of dental pulp on type I collagen
The purpose of this study was to regenerate human dental pulp tissues similar to native pulp tissues. Using the mixture of type I collagen solution, primary cells collected from the different tissues (pulp, gingiva, and skin) and NIH 3T3 (1 × 10 cells/ml/well) were cultured at 12-well plate at 37°C for 14 days. Standardized photographs were taken with digital camera during 14 days and the diame...
متن کاملInduction of type I collagen and osteocalcin in human dental pulp cells by retinoic acid.
Retinoic acid has been known to play a key role in the regulation of bone cell differentiation and function. The effects of retinoic acid on human dental pulp cells, which contain several characteristics similar to those of bone cells, has yet to be elucidated extensively. The effects of retinoic acid on human dental pulp cells in terms of type I collagen and osteocalcin induction were investig...
متن کاملEnhanced Dentin-Like Mineralized Tissue Formation by AdShh-Transfected Human Dental Pulp Cells and Porous Calcium Phosphate Cement
The aim of the present study was to investigate the effect of Sonic hedgehog (Shh) on human dental pulp cells (hDPCs) and the potential of complexes with Shh gene modified hDPCs and porous calcium phosphate cement (CPC) for mineralized tissue formation. hDPCs were cultured and transfected with adenoviral mediated human Shh gene (AdShh). Overexpression of Shh and cell proliferation was tested by...
متن کاملFGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells.
We investigated the effects of both cytokines and extracellular matrices on the proliferation and differentiation of immature adult rat incisor dental pulp cells. These immature cells, which have a high-proliferative potency in vitro and do not express mRNAs for dentin non-collagenous proteins such as dentin sialoprotein (DSP), bone sialoprotein (BSP), and osteocalcin, exist in the root regions...
متن کاملHuman dental pulp stem cells produce mineralized matrix in 2D and 3D cultures
The aim of this study was to characterize the in vitro osteogenic differentiation of dental pulp stem cells (DPSCs) in 2D cultures and 3D biomaterials. DPSCs, separated from dental pulp by enzymatic digestion, and isolated by magnetic cell sorting were differentiated toward osteogenic lineage on 2D surface by using an osteogenic medium. During the differentiation process, DPSCs express specific...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Oral Biology
سال: 2000
ISSN: 0385-0137
DOI: 10.2330/joralbiosci1965.42.102